Experimental advantages of coomassie brilliant blue method

(1) has a high sensitivity, which is estimated to be about 4 times higher than Lowry's method, and its minimum protein detection amount can reach 1mg. This is because the color changes greatly when protein is combined with dye, and the extinction coefficient of protein-dye complex is higher, so the change of light absorption value with protein concentration is much greater than Lowry method.

(2) The determination is quick and simple, and only one reagent is needed. It only takes about 5 minutes to complete the determination of a sample. Because the process of combining dye with protein can be completed in about 2 minutes, its color can be kept stable within 1 hour, and the color stability is the best between 5 minutes and 20 minutes. So there is no need to control time as time-consuming and strict as Lowry method.

(3) Less interfering substances. For example, K, Na, Mg2 ions, Tris buffer, sugar and sucrose, glycerol, mercaptoethanol, EDTA, etc., which interfere with Lowry method, do not interfere with this method.