What are the methods for cultivating viruses?

Virus culture methods include animal inoculation, chicken embryo culture, tissue culture, and cell culture.

1. Animal vaccination: After the virus enters the body of susceptible animals through injection, oral administration, etc., it can multiply in large quantities and cause the animals to produce specific reactions. Advantages: Easy to operate. Disadvantages: Affected by the body's immunity, sterile animals are often required. Production of vaccines and antisera.

2. Chicken embryo culture: Generally, 9-12 day old chicken embryos are used and inoculated into the yolk sac, amniotic cavity, allantoic cavity and other parts. Used for virus isolation and vaccine production.

3. Tissue culture: A method of cultivating viruses on living cells in vitro. Tissue block culture: Take tissue pieces for culture. Cell culture: After viruses infect cells, most of them cause cytopathic effects, which is called the cytopathic effect of viruses. It manifests as cell deformation, granulation in the cytoplasm, nuclear condensation, and nuclear lysis.

4. Use the full culture medium of the cell where the virus is parasitic to culture the cell. After culturing for a period of time, put in the specific virus and then culture it. Note that if the cell is an animal cell, the culture medium Add serum.