What is the most commonly used second generation sequencing technology at present?

Second-generation sequencing is a technology, but there are several sequencing principles. One is the principle of illumina sequencing while synthesizing, which is widely used at present, and the sequencing method of H- ion conversion ph of life.

The existing technical platforms mainly include Roche /454 GS FLX, Illumina/Sol-exa GenomeAnalyzer and Helicos BioSciences? Single molecule sequencer, rotator; Introduced by Dana-her Motion Company of the United States;

And sequencing by ligation, that is, locating nucleic acid information by primers. Technology platform is applied biological system/solid? System.

Extended data:

The background of the second generation sequencing technology;

1, DNA sequencing technology:

For a long time, DNA sequencing technology has been one of the most commonly used technical means in molecular biology related research, which has promoted the rapid development of this field to some extent. Human genome project, transcriptome analysis, microbial genome resequencing and single nucleotide polymorphism (SNP) analysis have also promoted the research and development in other biological fields.

The upgrading of each generation of sequencing technology marks a new breakthrough in the fields of gene chip, data analysis, surface chemistry and bioengineering in biology, and its application in the field of sequencing greatly reduces the sequencing cost, improves the sequencing efficiency, and makes sequencing develop towards Qualcomm, low cost, high security and commercialization.

2, the first generation of DNA sequencing:

Although the first generation of DNA sequencing technology has helped people to complete a lot of sequencing work with its reading length of 1000 bp and high accuracy rate of 99.999%, its shortcomings such as slow testing speed, high cost and low Qualcomm also hinder its wide application.

With the progress of science and technology and the efforts of researchers to develop sequencing technology, the 454 sequencing system released by Roche in 2005 marked the arrival of the era of parallel sequencing in Qualcomm.

Second-generation DNA sequencing technology, also known as massive parallel sequencing (MPS) and High-Throughput Sequencing (high-throughput sequencing), can rapidly sequence hundreds of thousands to millions of DNA molecules in hundreds of samples at the same time, with low cost and over 99% accuracy.

The representative technologies in this period are Roche 454, Illumina Solexa and ABI solids. Because the sequencing technology in this period was very advanced, the market was mainly monopolized by these three companies.