Brief introduction of lentinan

Directory 1 Pinyin 2 English reference 3 Overview of Lentinus edodes 4 Pharmacological Action 5 Lentinus edodes Drug Standard 5. 1 Official Name 5.2 Chinese Pinyin 5.3 Standard No.5.4 Latin or English 5.5 Main Active Components 5.6 Characters 5.7 Identification 5.8 Inspection 5.9 Content Determination 5. 10 Function and Usage 5.1/KLOC-. Usage and dosage 5. 12 note 5.66 6.2 English name 6.3 lentinan alias 6.4 classification 6.5 dosage form 6.6 pharmacological action 6.7 pharmacokinetics 6.8 indications 6.9 contraindications 6. 10 precautions 6.1adverse reactions 6 of lentinan Usage and dosage of kloc-0/2 lentinan 6. 13 interaction between lentinan and other drugs 6. 14 experts

2 English reference lentinan

3 Overview of Lentinus edodes Lentinus edodes is from traditional Chinese medicine Lentinus edodes (Berk. ) sing. It is a kind of dextran combined with 1β→3. It exists in the fruiting body of shiitake mushrooms. ) sing. The molecular weight is about 654.38+00000.

4 pharmacological effects lentinan has anti-tumor activity, which can be achieved by enhancing immunity or as interferon inducer. S 180 has a high inhibitory rate on mice.

5. Lentinus edodes drug standard 5. 1 officially named Lentinus edodes.

5.2 Chinese Pinyin Gu Xiang Duotang

5.3 standard number WS29 1(X256)97

5.4 Latin or English lentinan

5.5 Extract refined polysaccharide from the fruiting body of Lentinus edodes, and its main active components. The content of lentinan shall not be less than 92.0% in terms of dry product.

5.6 White or light yellow powder; Odorless moisture absorption.

Almost insoluble in water, methanol, ethanol or acetone; Dissolved in 0.5 mol/L sodium hydroxide.

Take this product for specific optical rotation, put it in a phosphorus pentoxide dryer, dry it under reduced pressure to a constant weight, accurately weigh an appropriate amount, add 0.5mol/L sodium hydroxide solution to dissolve it, quantitatively dilute it into a solution containing 10mg per 1ml, and determine it according to law (China Pharmacopoeia

5.7 Identification (1) Take 2ml of the solution under the content determination, add 5ml of anthrone solution (take 35mg of anthrone, put it in a 100ml volumetric flask, dissolve it with sulfuric acid, and dilute it with sulfuric acid to obtain a standby preparation), shake it evenly, and heat it in a water bath, which should be blue-green.

(2) Take about 10mg of this product, add a little water for grinding, then add water to make a solution containing 0.5mg per 1ml, and put it in a homogenizer to make a homogeneous slurry. Take 10ml, add 4.28 g of sodium periodate (NAIO 4) and 0.005mol/L of sulfuric acid solution. Dissolve and dilute to 1000ml] 1ml, shake well, immediately take 4ml of reaction solution, take water as a blank control, and measure the absorbance (A 1) at the wavelength of 295nm according to spectrophotometry (China Pharmacopoeia 1995 Edition, Appendix IV A), and place the remaining reaction solution in a shelter. After continuous stirring at 30.d egree. C. for 6 hours, it was taken out and its absorbance was measured (A2). The difference between the two absorbances (A 1A2) should be 0. 15 ~ 0.25.

(3) The infrared absorption spectrum should be consistent with the reference substance, and there is a weak absorption peak near 89Ocm 1.

5.8 Check the pH value. Take this product, add water to make 0.05% slurry, and measure it according to law (China Pharmacopoeia 1995 Edition, Part II, Appendix VI H). The pH value should be 6.0 ~ 8.0.

Intrinsic Viscosity Take this product, put it in a phosphorus pentoxide dryer, dry it under reduced pressure to constant weight, accurately weigh an appropriate amount, add a few drops of 0.5mol/L sodium hydroxide solution to make it fully swell, grind it evenly, leave it at 25 ~ 30℃ for 6 ~ 8 hours, make it completely dissolved, make a solution containing 0.5mg per 1ml, shake it evenly, and determine it according to law (China Pharmacopoeia 6544

Loss on drying takes a proper amount of this product, puts it in a phosphorus pentoxide dryer, and dries it under reduced pressure to a constant weight, and the weight loss shall not exceed 3. 5% (China Pharmacopoeia 1995 Edition II Appendix VIII L).

5.9 Preparation of Control Solution for Content Determination Take 25mg of Lentinus edodes reference substance, weigh it accurately, add 0.5mol/L sodium hydroxide solution 100ml, grind and dissolve it, add water, transfer it to 1000ml volumetric flask, dilute it to scale, and shake well.

Preparation of test solution Take 25mg of this product, weigh it accurately, and prepare the control solution according to the method under "Add 0.5mol/L sodium hydroxide solution ……".

Determination method Accurately measure 2.5ml of control solution and 2.5ml of test solution respectively, slowly add them into a test tube filled with 5ml of anthrone solution along the tube wall to make them layered (all the above operations are carried out in an ice bath), and immediately put them into a boiling water bath for heating after shaking and mixing, and start shaking for 6 minutes, and the reaction is accurate. Move the test tube to an ice bath to cool, and then leave it at room temperature. After shaking, take water as a blank, and determine the absorbance within 20 ~ 40 minutes at the wavelength of 625nm by spectrophotometry (China Pharmacopoeia 1995 Edition, Part II, Appendix IV), and then calculate.

Preparation of sulfuric acid solution of anthrone Take 35mg of anthrone (analytically pure), put it in a 100ml volumetric flask, add sulfuric acid to dissolve it, and dilute it with sulfuric acid to get the final product. Ready for immediate use.

5. The function and use of10 as an adjuvant drug for cancer.

5. 1 1 Usage and dosage 5. 12 Note 1. At present, there is no clinical experience for infants, so infants should use it with caution.

2. Pay attention to the possibility of allergic reaction.

3. After adding the solvent, shake it vigorously to dissolve it completely, and use it immediately.

4. Use according to the doctor's advice.

5. 13 dose intravenous drip or intravenous injection, once 1mg, twice a week.

5. 14 labeled amount 5. 15 Category 5. 16 preparation is administered intravenously once 1mg twice a week.

5. 17 Specification 5. 18 Store tightly in a dry place.

5. The validity period of19 is tentatively set at two years.

6 Lentinus edodes instructions 6. 1 drug name Lentinus edodes

6.2 English name lentinan

6.3 Lentinus edodes alias Lentinus edodes; Lentinus edodes polysaccharide; Tumor blocking energy; Heaven and earth are happy; Can cure difficulties

6.4 classification of digestive system drugs >; Auxiliary drugs for liver disease

6.5 dosage form 1. Tablet: 2.5mg；;

2. Injection: 2mg (2ml).

6.6 The pharmacological action of lentinan can enhance immunity. Although its mechanism of action does not directly kill tumor cells in vivo, it can exert anti-tumor activity by enhancing the immune function of the body. In vivo, it can enhance the activity of NK cells in spleen and abdominal cavity. The induced interferon is related to the dose of lentinan, and its activity has synergistic effect with interleukin or interferon inducer. It is also proved that lentinan can enhance the anti-HIV activity of deoxythymidine in vitro. The combination of lentinan and antitumor drugs can enhance the sensitivity, especially when combined with FT207, the survival time of experimental animals is significantly longer than that of FT207 alone.

6.7 Pharmacokinetics of Lentinus edodes The pharmacokinetics of Lentinus edodes in vivo is similar to that of polysaccharides such as dextran. After administration to normal mice, rats and dogs, the blood concentration decreased rapidly and then slowly, showing a biphasic change. After 5 minutes, shiitake mushrooms are mainly distributed in liver, spleen, lung and kidney, and their distribution in vivo is the same as that of single administration, and they are mainly excreted through urine. After normal animals were given lentinan, the blood concentration was biphasic, mostly distributed in liver, followed by spleen, lung and kidney. The uptake of lentinan by tumor tissue is not specific. Mainly excreted with urine, a small amount with feces, almost can not pass through the placenta. It will not enter the milk.

6.8 indications of lentinan 1. Mainly used in combination with radiotherapy, chemotherapy and surgery for gastrointestinal tumors that are not suitable for surgery or recurrence. Lentinan plus radiotherapy and chemotherapy for small cell lung cancer, breast cancer and malignant lymphoma. It can also be used to treat cancerous hydrothorax and ascites.

2. Antivirus: It can be used for hepatitis B and acquired immunodeficiency syndrome (AIDS).

3. Anti-infection: it is used for drug-resistant pulmonary tuberculosis and also for the treatment of chronic bronchitis in the elderly.

4. It is used as adjuvant therapy for chronic hepatitis and digestive tract tumor.

6.9 contraindications of lentinan are prohibited in patients with rheumatic heart disease.

6. 10 Precautions 1. Use with caution for allergic people.

2. Pregnant women, lactating women and children should use it with caution. Lentinus edodes polysaccharide should be used immediately after being dissolved in physiological saline or 5% glucose injection, and should not be stored for a long time.

Adverse reactions of lentinan 1. Some patients sometimes have anorexia, nausea, vomiting, chest tightness, shortness of breath, headache, dizziness, rash, sweating, fever and slight pain at the muscle injection site. Can be symptomatic treatment, severe cases can be stopped.

2. Leukopenia and hemoglobin reduction are rare, and anaphylactic shock is also rare. Special attention should be paid to chills, irregular pulse, decreased blood pressure, abnormal oral sensation and dyspnea. People with anaphylactic shock should stop taking medicine immediately and give first aid.

6. 12 Usage and dosage of lentinan 1. (1) orally, 4-5 tablets each time, twice a day, and 3 months is 1 course of treatment; (2) intramuscular injection: 2 ~ 4 mg per day for 10 ~ 20 days; (3) Intravenous injection: 1 ~ 2 mg each time, 1 ~ 2 times a week or as directed by the doctor. At the same time, oral administration of FT207,400 mg/m2, or intravenous infusion of FT 207, 0.6 ~ 1g each time.

2. Cancerous hydrothorax and ascites: 4mg lentinan is dissolved in 10ml physiological saline, once a week, and given intraperitoneally for ***4 times. At the same time, ft 207 400 ~ 800mg is given orally or intravenously, once a day.

3. Treatment of hepatitis B and AIDS, 4mg, intramuscular injection, daily 1 time, 8 weeks for 1 course of treatment.

6. 13 drug interaction When lentinan is compatible with vitamin A preparation, the injection will be turbid, so compatibility should be avoided.

6. 14 expert comments: The application in drug-resistant pulmonary tuberculosis can stop phlegm, improve patients' symptoms, and strengthen the phagocytosis and conditioning of neutrophils on tuberculosis. Lentinus edodes polysaccharide is an immune enhancer. It is one of the auxiliary drugs for chronic diseases such as chronic hepatitis and digestive tract tumors. Can enhance the body's cellular immunity and humoral immunity, and improve the body's disease resistance.

Xiangyun Gantai Granule B09689 1 Prescription: Lentinus edodes and Coriolus versicolor dry extract fine powder (3:2) and appropriate amount (equivalent to 28g of proteoglycan) Preparation method: Take Lentinus edodes and Coriolus versicolor. ...

Xiangyun gantai tablets