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Can liquid nitrogen preserve organs safely and without damage?
It is absolutely possible, but it is very difficult. A girl’s mother in China passed away, and it cost $500,000 to have her head frozen in the United States. If you just count the cost, for 50 years of liquid nitrogen, a 100L liquid nitrogen tank is enough. The 200L tank is much richer and made into a special shape. Add liquid nitrogen every year. This liquid nitrogen cannot be bought. There is a liquid nitrogen machine that can make it. The cost is very low; even if the liquid nitrogen tank needs to be replaced 10 times in 50 years, it will cost US$50,000. Why does it cost $500,000? Because people’s requirements are very high! From what I learned just by reading the news, starting from the first minute after death, cleaning fluid is injected, the blood in the head is replaced, the head is removed, and the water in the head is removed as much as possible. What ingredients are added to the cryopreservation solution? What ratio? Which blood vessels are added to replace the blood in which tissue? How many times have you joined? How many times did you take the time? How much blood and water must flow in and out to be considered qualified? What kind of liquid needs to be added to preserve it before refrigeration? It will then be transferred to the United States for long-term freezing. The monitoring during the freezing process even puts forward very strict requirements on how many degrees it should be reduced per second. Even many academicians of the Chinese Academy of Sciences would not make such detailed requirements!
If you mention this statement to some doctors, they will laugh at you because they think it is unnecessary and their teachers have never mentioned it. Many people laugh at the ridiculous idea of ??Americans, saying that they want to be a thousand-year-old king and an eighty-thousand-year-old tortoise, freezing hundreds of thousands of dollars and wasting millions of dollars. . . The reality is not that the operating cost is high, but that this process requires professional knowledge, professional talents and precise instruments to complete. There are few companies in the world that can do this process in such detail, so the price is high! The data and experience summarized from a large number of experiments will also be of great help to them in improving their technology in the future! Take the microscope as an example. The phase contrast microscope was invented by the Dutch. German and Swiss scientists invented the tunnel scanning microscope and both won the Nobel Prize! Especially the tunnel scanning microscope can make the structure inside the cell look like a cartoon, but the Chinese can only buy it! This time, the cryo-electron microscope was invented by someone else. Shi Yigong said it was really good. Let’s keep buying it! Unfortunately, the price of cryo-EM is too high, and many national key laboratories in 985 key universities cannot afford it! Zhejiang University bought one for 60 million yuan and used it all for publicity! Shi Yigong and others even went to show their support, which is really enviable! ! !
As you can see below, it is mentioned that organs will suffer from frostbite when they are thawed, because the laboratory conditions of Chinese masters and doctors are very simple, and the laboratory and storage room are in the same place. Close the door, put on dust-free clothes, put on gloves, and place a clean workbench to call it a sterile room. A few meters away is a warehouse with people coming and going. . . Take it out and throw it directly into the water bath according to the method taught in the school. This will inevitably involve a considerable risk of failure! When he failed, he just said one word and it was all like this. Only international-level scientific research companies conducting special thawing experiments will strictly require every step. This is like setting up an anesthesiology department in a hospital as a separate department; an anesthesiologist in a small village said, "Isn't it enough to just give an injection?"
If you want to ask questions in this regard, it is recommended that you go to large libraries to find some foreign experts who specialize in frozen books instead of asking people online. Several books could be written about freezing and thawing. If the professor takes out a chapter and translates it, improves it, then it can be included in his college textbook. I definitely don’t need so much knowledge, but it doesn’t mean that I think what I’m doing is right, but my requirements are not that high, and I don’t have the conditions. The more you study biology, the closer it is to physics/mathematics/chemistry/computers, and the more unfathomable it becomes!
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