Do nucleic acid test is nose or throat nucleic acid test sampling is nose?

As we all know, nucleic acid detection is a commonly used diagnostic method, which can judge whether novel coronavirus is infected in a short time, which is very helpful for virus prevention and control. Many people will have a nucleic acid test. So is it nose or throat to do nucleic acid test? Let's take a closer look!

Whether the nucleic acid test is nose or throat may be different in every place, and it is needed in most areas, such as Shanghai and Suzhou. In some places, just take a sample from the throat. There are procedures for operation, and the problem is not big. Consult the attending doctor specifically.

In order to ensure the accuracy of detection to the greatest extent, the sampling point needs to take two nasal swabs and one throat swab samples from the subject, and send them to the testing institution by a special logistics organization, and the results will be obtained within 24 hours.

Whether collecting nasopharyngeal swab or oropharyngeal swab, the depth of collection is the key. The collected nasopharyngeal swab did not reach the depth of nasal cavity, and most of the cells that may be collected are virus-free cells, which may cause "false negative".

The correct operating procedure of nasopharyngeal swab is 1. The sampler holds a cotton swab (a plastic stick cotton swab with polypropylene fiber head) in his right hand and holds it on the top of the subject's head in his left hand.

2. Make the cotton swab with a slightly downward bending tip enter from the front nostril and slowly penetrate backward along the bottom of the lower nasal passage. Because the nasal passage is curved, the movement should be gentle and slow, and the force should not be too strong to avoid traumatic bleeding.

3. When the tip of the cotton swab reaches the back wall of nasopharyngeal cavity (touching the wall), leave the cotton swab for a moment (about 3 seconds), then gently rotate it for one week and slowly take it out.

4. Collect another nostril with another 1 plastic cotton swab with polypropylene fiber head in the same way, immerse the above two cotton swabs in the same tube with 3ml virus sampling solution, discard the tail and tighten the tube cover.

5. After packaging according to the requirements of biosafety, it should be transported to the laboratories of CDC at all levels for virus nucleic acid detection as soon as possible.

How many times should the nucleic acid be tested? In general, nucleic acids should be checked at least twice with an interval of at least 24 hours.

Novel coronavirus nucleic acid can be detected in nasopharyngeal swab, sputum and other lower respiratory secretions, blood, feces and other samples, but in order to improve the positive rate of nucleic acid detection, it is suggested that sputum should be taken as much as possible. If it is a patient with tracheal intubation, lower respiratory secretions can be collected and sent for inspection as soon as possible after collection. In addition, according to the current cases of coronavirus, in addition to checking nucleic acid, we should also check the symptoms, signs and other laboratory tests of the patients, such as the normal or decreased total number of white blood cells in peripheral blood, the decrease of lymphocyte count, and the serious increase of D- dimer in some patients. Chest imaging examination can show that there are many small patches and interstitial changes in the early stage, especially in the outer lung, and then develop into multiple ground-glass shadows and wet shadows in both lungs, and even lung consolidation in severe cases.

How long does it take for nucleic acid detection to produce results? Generally, the results can be produced within one day.

Nucleic acid detection is the diagnosis of clinical etiology by analyzing the DNA or RNA genetic sequence of pathogenic microorganisms under laboratory conditions. This method of nucleic acid detection is detected by PCR amplification, which requires many steps, and it takes 6 hours at the earliest to produce results. This kind of detection has high sensitivity, high cost, completely manual operation, very heavy workload and very high requirements for laboratory conditions. It is the most effective detection method to determine whether the patient is infected with the virus as soon as possible, and to find and treat it as soon as possible.