Must the tissue culture experiment of plants be completed in a professional laboratory?

Several factors of success in tissue culture experiment

One. Experimental environment

Specialized plant tissue culture laboratory is one of the key factors for the success of plant tissue culture. The whole process of plant tissue culture must be carried out in a sterile environment, from the disinfection, pruning and inoculation of explants to the later aseptic seedling raising, it is inseparable from a sterile experimental environment. A professional and clean plant tissue culture laboratory is an indispensable condition for the success of plant tissue culture experiment, which can greatly reduce the probability of pollution during tissue culture operation and aseptic seedling culture.

Second, experimental equipment and consumables

1. clean workbench: it is a general air purification equipment, which can provide a local high-cleanliness working environment. It is the core instrument in plant tissue culture and can provide 100 cleanliness. Without the sterile environment provided by clean workbench, the success rate of plant tissue culture is almost zero. In the conventional laboratory environment, it is impossible to carry out plant tissue culture operation. Even if the experimental operation is completed, the finally cultivated aseptic seedlings will be contaminated by external microorganisms. Chuanbulan clean workbench is a national patent protection product with anti-ultraviolet injury device. This device can prevent students from being hurt by ultraviolet rays when they forget to turn off the ultraviolet lamp for experiments due to improper operation, and it is an essential "weapon" for successful plant tissue culture.

2. Autoclave sterilizer: It is another core instrument of plant tissue culture experiment, which sterilizes the culture medium and inoculation instrument in the process of plant tissue culture to achieve the purpose of sterility. In the 2065438+February Beijing Chuanblan Tissue Culture Competition, some schools did not buy autoclaves, but used ordinary household pressure cookers to sterilize the culture medium (the sterilization temperature of the household pressure cookers failed to reach the temperature of completely killing bacteria). Finally, the experimental results show that the probability of pollution is above 70%, so the tissue culture competition cannot be completed. However, most schools that use autoclave sterilization medium combined with clean workbench can effectively control the pollution probability below 10%, and some schools even achieve zero pollution. In a word, clean workbench and autoclave are decisive instruments for the success or failure of plant tissue culture, and choosing professional essential instruments for tissue culture is more conducive to the development and success of the experiment.

3. Micro-adjustable pipettes and suction heads: experimental tools for measuring micro-liquid, which are used to measure micro and micro hormone mother liquor and adjust the hormone concentration required in various culture media. The application rate of fine-tuning pipettes in other experiments is also very high. Some experiments require that the instruments used should be sterile, that is, they can be sterilized at high temperature and high pressure and irradiated by ultraviolet rays. Traditional pipettes can't be completed, but the fine-tuning pipette developed by Chuanblan fully considered this point in the design and development.

4. Illumination culture rack: the place where aseptic seedlings are cultivated, which provides the light needed for plant growth, and the light intensity can be controlled according to the actual situation. If the inoculated tissue culture bottle is directly placed in the general environment, the induction will fail due to insufficient light intensity. Therefore, it is best to choose light culture frame to provide more suitable conditions for tissue culture seedlings.

5. Illumination incubator: a place for cultivating aseptic seedlings, which can not only provide appropriate light intensity, but also control the temperature of aseptic seedlings during plant cultivation. In addition, the light incubator can also adjust the light cycle according to the growth habits of different plants, which is suitable for extracurricular exploration activities. For example, short-day chrysanthemum varieties can control chrysanthemum flowering or raise insects and small animals by adjusting light time. Light incubator is more powerful than light incubator, its application is not limited to the scope of plant tissue culture, and its management is more convenient. Even in the case of unattended in winter and summer, as long as all parameters are set in advance, there will be no worries.