How to upload the functional genes of bacteria to NCBI to obtain GenBank number?

Previously, we have explained how to upload the sequenced and spliced bacterial 16S rDNA sequence to NCBI to obtain the GenBank number:

Step of uploading NCBI in 16S sequence

In fact, the sequence of uploading bacterial functional genes to NCBI to obtain GenBank number is basically the same as uploading 16S sequence, and the main differences are as follows (taking uploading gyrA gene as an example):

The file uploaded by gyrA gene needs to prepare two documents-the gene sequence of gyrA gene and the protein sequence of Gyra gene, in which the protein sequence is the translation sequence of the gene sequence and the protein sequence must be continuous, with no spaces or underscores in the middle (if underlined, it is necessary to add or subtract bases or complementary sequences of the translation sequence).

The entry of gyrA gene needs to select the second window in the submission, and then select Start? BankIt submission options.

? After entering, start to fill in personal information, and then fill it out step by step according to the prompts. Please refer to the video above or the previous article to fill in the rules (note: you need to click to continue twice to fill in the nucleotide and organization). During submission, provide information in the Source Modifier option, such as the source of the submission sequence. If it is a gene on the genome, just fill in it directly according to the following figure (you need information verification after clicking Continue). The next step, Features, is the biggest difference between the whole submission process and 16S submission-you need to provide the protein sequence encoded by the submission sequence, which can be submitted in two ways: 1) upload the FASTA format text prepared in advance; 2) Fill in online.

? Here we choose to submit online, click the option of adding features by filling in the input form, select the option of CDS, then select the option of providing protein sequence data below, and then click Add to open a new interface. The new interface needs to fill in at least the information shown in the figure below (the ID of the nucleic acid sequence displayed in the TXT text must be consistent with the protein sequence). Finally, we can submit the information after verification. After submission, we will wait for the processing to be completed, and they will send you the GenBank number by email.