Who can tell me the difference between Illumina 454 ABI sequencing?

First of all, in principle, Illumina sequencing chip uses bridge amplification to form "clusters", terminator method, and bidirectional sequencing; 454 uses magnetic bead method to amplify, using the polymerization reaction by-product PPi for subsequent reactions. , to obtain fluorescence; ABI's SOLiD system also uses magnetic bead amplification, but uses a ligation reaction (the specific method is slightly complicated).

Secondly, in terms of performance, the throughput of Illumina sequencers is extremely high. The current HiSeq Based on the characteristics of reaction sequencing, its accuracy should be the highest.

Finally, let’s take a look at their shortcomings. Illumina is expensive, especially the later extremely high-throughput sequencers that ordinary laboratories cannot afford. At the same time, their instruments may be supervised, and they are designated to only sequence certain biological samples. Of course, the price/performance ratio is still there; the shortcoming of the 454, if only compared among these three, is that its homopolymer accuracy is biased. Low; for SOLiD, the read length is extremely short. At the same time, due to the sequencing principle of SOLiD, the sequencing system is relatively complex, so the SOLiD system is not easy to upgrade. It seems that the SOLiD system is rarely heard of now.