How to plant Agrocybe aegerita? What problems should be solved? Please also have friends who understand and talk to me!

Agrocybe chaxingu is a wood rot fungus, and its ability to decompose lignin and cellulose is weak. Wild Agrocybe aegerita only grows on camellia oleifera. After artificial domestication, broad-leaved trees such as tung tree, maple tree, willow tree, oak tree and poplar can be used as cultivation materials, but sawdust with loose materials and less tannin content is more suitable for the growth of Agrocybe aegerita. Bran, rice bran and cake fertilizer can be fully utilized as nitrogen sources.

Agrocybe aegerita has no activity of insect laccase, and its utilization ability of lignin is weak. However, the protease activity is very strong, and the ability to use protein is correspondingly strong. According to this characteristic of Agrocybe aegerita, we designed two groups of formulas:

1. sawdust 30%, cottonseed hull 36%, tea seed cake powder (rapeseed cake powder, peanut cake powder, etc. ) 5%, wheat bran 23%, corn flour 5%, gypsum 1%.

2. Mushroom powder 50%, cottonseed hull 20%, tea seed cake powder (rapeseed cake powder, peanut cake powder, etc.). ) 5%, wheat bran 19%, corn flour 5%, gypsum 1%.

Agrocybe aegerita mycelium has alkali resistance. Adding 2-3% quicklime to the formula can effectively promote the growth of Agrocybe aegerita mycelium and inhibit the spread of miscellaneous bacteria.

Cultivation bag making

1. Mix the cottonseed hull according to the formula, then mix it with other raw materials evenly, then add water and mix well. The water content is controlled at 60-65%, and the degree is that the culture material is grasped tightly, and there is a watermark between the fingers but it does not drip.

2. Bag with 15- 17㎝×33㎝ bacteria bag, bag tightly, then tie the bag mouth tightly with a string (replace the sterilized collar and cotton plug when inoculating), or seal it with a sponge cover.

The mycelium growth of Agrocybe chaxingu is slow. In order to improve the yield, high-quality mushroom bags or double-layer bags should be selected.

disinfect

1. Sterilization at atmospheric pressure will be heated to 100℃ and kept at 12- 16 hours. It is not allowed to stop fire and cool down halfway, otherwise the sterilization time will be prolonged.

2. Autoclave with strong fire to 0.05Mpa, and open the air valve to discharge cold air to avoid false air pressure and incomplete sterilization. Then use strong fire to 0.15mpa for 2 hours.

After sterilization, the culture bag can be moved to a pre-sterilized place for cooling, or directly moved to the inoculation room for cooling.

vaccination

1. Spray the inoculation box or inoculation room with 5% Lysol solution.

2. Move into a cooled culture bag (the material temperature is below 30℃) and an inoculation tool.

3. Disinfect the inoculation box (room) with aerosol disinfectant, the dosage is 3-4g/m3 (Agrocybe aegerita mycelium is sensitive to disinfectant, too much dosage will cause "dead species", too little will easily infect miscellaneous bacteria, leading to inoculation failure). Inoculation box (room) should be sealed.

4. Soak Agrocybe aegerita strains with 0.25% bromogeramine solution, and then bring them into the inoculation box (room) for aseptic inoculation. The method is similar to other edible fungi. Because of the poor anti-impurity ability of Agrocybe aegerita mycelium, in order to gain group advantage and improve the finished product rate, the inoculation amount should be increased, and it is appropriate to have 20-30 bags per bottle or 30-40 bags per bag.

Mycelial culture

After inoculation, the culture bag is placed in a ventilated and cool place for culture, the feed temperature is controlled at 20-28℃, and the relative humidity of air is controlled at 55-75%, so that the hyphae grow in the bag for 40-50 days, and fruiting occurs in 55-65 days.

Mushroom production management

1. Vertical bag cultivation: take off the collar and cotton plug from the physiologically mature cultivation bag (after the mycelium is full 10- 15d), put them on the border in the mushroom shed, arch the film to moisturize and accelerate germination, and adjust the temperature 16-26℃ and air relative humidity. After 10- 15d, a large number of mushroom buds appeared. The film at the mouth of the bag can be cut off, the humidity can be adjusted to 85-95%, and the CO2 concentration is lower than 0.3%, so that the mushroom buds can continue to develop. After picking the first mushroom, remove the remaining mushroom feet, replenish water, and further grow mushrooms. Conditional can engage in indoor layered stereo cultivation.

2. Wall cultivation: Take off the collar and cotton plug of the physiological mature cultivation bag and pile them in the frame or indoors (preferably) to form a wall (6- 10 layer), and the rest are the same as above. This method saves space, but there are more deformed mushrooms.

3. Weathered soil and garden soil with loose soil should be selected, sprayed with 1% formalin, covered with film and sealed for more than 3 days, then the film is uncovered to let the formalin disperse, and then 2% slaked lime, 1% calcium peroxide and 0.2% urea are added and mixed well. In addition, 1% calcium peroxide and 0.2% urea can also be added to the burnt soil, which can be used as the covering material after being evenly mixed. Then move the physiologically mature cultivation bag to the mushroom farm disinfected with lime and insecticide in advance, take off the bag, arrange it horizontally, and cover it with soil 1-2㎝ (for outdoor cultivation, cover it with bamboo chips and film). 10d later, spray them to keep the soil moist and wait for the mushrooms to grow. Other methods are basically the same as above, but the number of water sprays and the amount of water can be reduced appropriately. The first two methods can also be used to remove bags and cover the soil to produce mushrooms in the later stage.

Agrocybe aegerita mycelium has poor stress resistance and rich nutrition, which is a hotbed of various diseases and insect pests. In the process of cultivation, we should pay attention to the following two points:

(1) Pay attention to aeration, prevent high temperature and humidity, inhibit the growth of miscellaneous bacteria, and create an environment conducive to the growth of Agrocybe aegerita.

(2) High-efficiency and low-toxicity pesticides should be used in time. Generally speaking, highly effective pyrethroids can kill many kinds of edible fungi pests.

8. Agrocybe chaxingu is 8% mature, and the harvesting and processing period is before the membrane breaks. After harvesting, remove foreign bodies from the roots, dry or fresh needles.