Briefly describe the advantages and limitations of cell culture.

Cell culture technology refers to the growth of cells in vitro, during which cells no longer form tissues. A culture is a single cell or a group of cells. When cells are cultured, they must live in an artificial environment. Due to the change of environment, some other factors may affect the movement of cells, making the cell culture time longer, and subculturing cells may lead to simplification. The following small series takes you to understand the advantages and disadvantages of cell culture technology.

Advantages of cell culture technology

1. Cell culture technology can directly observe the life activities and morphological structure of living cells. It has been applied to genetics, cytology, experimental medicine, oncology and immunology.

2. Cell culture technology can directly observe the changes of cells and obtain images conveniently.

3. Cell culture technology can be used to study various cells. From low to high to human, from embryo to adult, from normal tissue to tumor cells.

4. Cell culture technology When observing and studying the cell condition, various technical methods such as phase difference, fluorescence, electron microscope, histochemistry and isotope labeling can be conveniently used.

5. Cell culture technology is not only an integral part of molecular biology and genetic engineering, but also its main research object.

6. Cell culture technology can be conveniently used in physical, chemical and biological experiments.

7. Cell culture can easily provide a large number of experimental objects with similar biological characteristics, and it is very economical and cheap.

8. Cell culture is one of the sources of materials for monoclonal antibody production and genetic engineering of biological products.

Shortcomings of cell culture technology

Tissue and cells must survive independently in artificial culture environment after separation. Although it simulates the internal environment, it is still very different from the internal environment. Therefore, when doing experiments with cultured cells, don't think that the artificial culture environment is equivalent to the in-vivo environment, and it is easy to get the same results as the in-vivo environment.