Is there a charge for nucleic acid testing?

There is a charge for nucleic acid testing.

For example, from May 6th, Hubei Province, novel coronavirus's nucleic acid detection and antibody detection items were temporarily included in the medical insurance catalogue, and they were paid according to Class B. The specific standards of medical insurance payment for nucleic acid and antibody detection items were determined by local conditions, but they should not be higher than the previous highest price limit of 65,438 yuan for nucleic acid detection and 50 yuan for antibody detection.

Specific price standard of Dalian, Liaoning Province: 60 yuan/item of novel coronavirus nucleic acid detection (except related detection reagents); 30 yuan/item of serum novel coronavirus antibody detection (except related detection reagents).

Extended data

Principle of novel coronavirus nucleic acid detection

The most commonly used method for detecting novel coronavirus-specific sequences is fluorescence quantitative PCR (polymerase chain reaction).

Because the template of PCR reaction is only DNA, novel coronavirus nucleic acid (RNA) should be reverse transcribed into DNA before PCR reaction. In the PCR reaction system, there are a pair of specific primers and a Taqman probe. The Taqman probe is a specific oligonucleotide sequence, and both ends of the probe are marked with a reporter fluorescence group and a quenching fluorescence group respectively.

When the probe is intact, the fluorescence signal emitted by the reporter group is absorbed by the quenching group; If the target sequence exists in the reaction system, the probe will bind to the template during the PCR reaction, and DNA polymerase will digest the probe along the template by using the nucleic acid exonuclease activity of the enzyme, and the reporter group will be separated from the quenching group to emit fluorescence. Every time a DNA strand is amplified, a fluorescent molecule is produced.

The fluorescence quantitative PCR instrument can monitor that the number of cycles (Ct value) of fluorescence reaching the preset threshold is related to the concentration of viral nucleic acid. The higher the concentration of viral nucleic acid, the smaller the Ct value. The products of different manufacturers will determine the positive judgment value of this product according to the performance of their own products.

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